1. Fully Automated Blood Group Serology

Biotest TANGO
Fully automated system for blood grouping, cross-matching, antibody screening, antibody differentiation and antibody titration.
Tango uses microtitre plates containing twelve individual strips of eight wells. It is these individual strips that progress through the analyser, thus optimising plate usage.Two types of methodology are utilised to achieve results. A straightforward haemagglutination method is used in the Erytype plates, with freeze dried antisera present on the wells. A solid phase method is used in the Solidscreen ll plates. Using these two technologies, a wide range of assays are available.

ERYTYPE

SOLIDSCREEN ll

2. Reagents for ABO and Rh Blood Grouping

3. HLA Serology

4. HLA DNA Typing
4.1 Biotest Elpha typing kit

The Biotest ELPHA HLA-AB and DR is a DNA-hybridization test for the determination of HLA-A and HLA-B and HLA-DR alleles. Sequence-specific oligonucleotide (SSO-) probes are used as diagnostic tools to identify polymorphic sequence motifs. The hybridization between probe and target DNA is detected by a method adapted from the protein ELISA technique. The microtest plate format allows the use of standardized apparatuses for automation of the test procedure and data evaluation. The test, however, also can be easily performed by hand.

Starting from genomic DNA which can be readily obtained from peripheral blood, the polymorphic region in the 2nd and 3rd exon of the HLA-A and HLA-B genes is amplified by means of the polymerase chain reaction (PCR) using Biotin-labelled primers. After amplification and denaturation of the PCR products the resulting biotinylated single strand molecules are bound to Streptavidin-coated wells of a microtest plate. The wells contain FITC-labeled SSO-probes in a dried form which redissolve when the diluted solution of the PCR products is transferred to the wells. Fixation of the PCR products to the wells and hybridization with the SSO probes thus occur in one step. The specificity of the assay is dependent on the subsequent stringent washing step in which probes of insufficient sequence homology to the PCR products are removed. Hybridization probes are visualized in a colour reaction involving FITC-specific antibody fragments coupled to Horseradish peroxidase (POD). The results are quantitated photometrically in an ELISA reader. The combination of HLA-A alleles and HLA-B alleles in the test material is determined from the pattern of positive reactions by means of the reaction schemes.

The Biotest ELPHA enzyme linked probe hybridization assay kits are intended for the determination of HLA Class I or Class II alleles.

SSP-Typing kits
Principle of the test

The Biotest SSPtray is a test system for typing of HLA-A,-B and –Cw and -DR characteristics using PCR techniques. For typing, the SSP method uses allele-specific primers in the amplification reaction. The method is based on the principle that only primers whose sequences are perfectly complementary to that of the target sequence of a DNA sample present will bind to this DNA and produce an amplificate in the PCR reaction. Non-complementary primers, on the other hand, do not bind to the DNA and no amplification takes place.

The amplified DNA is determined using agarose gel electrophoresis. Successful amplification will generate a DNA fragment of defined length which appears as a band. If no amplification takes place, this band is missing.

The composition of the primers permits positive identification of the HLA characteristics A*01-A*80, B*07-B*83 and Cw*01-Cw*18. The field of application for this detection system is the determination of individual HLA-A,-B and -Cw alleles of organ donors and recipients and of patients receiving blood transfusion or blood component substitution therapy.

Biotest HLA Antibody Screening and Determination ELISA Test Product line

The Biotest AbScreen HLA class II is a solid phase enzyme linked immunosorbent assay (ELISA). Microtiter plates are coated with highly purified HLA class I antigens from a large pool of human platelets. If the sample being tested contains specific antibodies against HLA class I, they will bind to the antigen in the wells of the microtiter plate. Unbound antibodies are removed in a washing step. The resulting antibody-antigen complex is detected using a specific enzyme-labelled antibody directed against human IgG (conjugate). The presence of bound antibodies is demonstrated by adding a chromogenic substrate PNPP which results in a coloured product. The reaction is stopped and interpreted by means of an ELISA reader.

The Biotest AbScreen HLA class II is a solid phase enzyme linked immunosorbent assay (ELISA). Microtiter plates are coated with highly purified HLA class II glycoproteins from EBV transformed B lymphocytes cell lines. If the sample being tested contains specific antibodies against HLA class II, they will bind to the antigen in the wells of the microtiter plate. Unbound antibodies are removed in a washing step. The resulting antibody-antigen complex is detected using a specific enzyme-labelled antibody directed against human IgG (conjugate). The presence of bound antibodies is demonstrated by adding a chromogenic substrate PNPP which results in a coloured product. The reaction is stopped and interpreted by means of an ELISA reader.

The Biotest AbIdent HLA class I is a solid phase enzyme linked immunosorbent assay (ELISA). Microtiter plates are coated with 40 different highly purified human HLA class I glycoproteins. If the sample being tested contains specific antibodies against HLA class I, they will bind to the antigen in the wells of the microtiter plate. Unbound antibodies are removed in a washing step. The resulting antibody-antigen complex is detected using a specific enzyme-labelled antibody directed against human IgG (conjugate). The presence of bound antibodies is demonstrated by adding a chromogenic substrate PNPP which results in a coloured product. The reaction is stopped and interpreted by means of an ELISA reader.

The Biotest AbIdent HLA class II is a solid phase enzyme linked immunosorbent assay (ELISA). Microtiter plates are coated with highly purified HLA class II glycoproteins from EBV transformed B lymphocytes cell lines. If the sample being tested contains specific antibodies against HLA class II, they will bind to the antigen in the wells of the microtiter plate. Unbound antibodies are removed in a washing step. The resulting antibody-antigen complex is detected using a specific enzyme-labelled antibody directed against human IgG (conjugate). The presence of bound antibodies is demonstrated by adding a chromogenic substrate PNPP which results in a coloured product. The reaction is stopped and interpreted by means of an ELISA reader.

5. Monoclonal Antibodies for Immunopathology and -virology

5.1 Monoclonal Antibodies for CMV Detection:

6. Biotest Infectious Disease Diagnostics

6.1 Biotest ELISA-Virology, recombinant:

7. Systems for Hygiene Control

7.1 Determination of the Microbial Content of the Air:
Biotest Air Sample RCS Standard
The standard RCS airsampler is a convenient instrument for measuring the concentration of air borne microorganisms. Possible investigations include

This instrument can be used in the following areas

Biotest Air Sample RCS Plus
The portable RCS PLUS centrifugal Air smpler can be used in all situations where pre-defind airborne microbial counts, whether based on legal requirements or on proprietary standards, must not be exceeded. The RCS Plus airsampler is suitable for the detection of bacteria and fungi, both cleanrooms and laminar flow equipment having microbial count limit values of ? 1 CFU/m3 (CFU =Colony Forming Unit) as well as areas with high microbial loading, such as composting plants or animal housing.

7.2 Determination of the Microbial Content in Liquids and on Surfaces

8. Airborne Particle Counter APC Plus

The compact APC 1000 Airborne Particle counter is suitable for the determination of particle concentrations in aereas in which defined concentration limits must not exceeded. The instrument provides rapid and accurate measurements ,particularly when searching forleaks in filtration equipment in clean rooms and workbenches with laminar flow extraction equipment.

9. Biotest Product list 2005


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